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acs 1020  (ATCC)


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    ATCC acs 1020
    Acs 1020, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acs+1020/ATCC-HYS0103+Human+Induced+Pluripotent+Stem+(IPS)+Cells/pm42044971-19-14-15
    Average 93 stars, based on 28 article reviews
    acs 1020 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxic in human brain organoids
    Article Snippet: 496 497 STAR METHODS 498 Detailed methods are provided in the online version of this paper and include the following: 499 • KEY RESOURCES TABLE 500 • RESOURCES AVAILABILITY 501 o Lead contact 502 o Materials availability 503 o Data and code availability 504 • EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 505 o Human iPSC generation and characterization – Reprogramming 506 o Human iPSC culturing 507 o Assessment of pluripotency 508 • METHOD DETAILS 509 o Cerebral organoids culture conditions 510 o Hypoxia modelling 511 o Immunofluorescence 512 o RNA extraction, RT-qPCR 513 o Shotgun proteomics 514 • QUANTIFICATION AND STATISTICAL ANALYSIS 515 o Proteomic Data Analysis 516 o Statistical analysis 517 518 519 Jo rna l P re- pro of 18 520 521 522 STAR METHODS 523 KEY RESOURCE TABLE 524 525 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SOX2 Abcam Cat #ab93689; RRID:AB_10562630. .. PAX6 Sigma-Aldrich Cat #AMAb91372; RRID:AB_2716656 NeuN Sigma-Aldrich Cat #MAB377; RRID:AB_2298772 MAP2 Sigma-Aldrich Cat #ZRB2290; RRID:AB_3677657 GFAP Sigma-Aldrich Cat #G3893; RRID:AB_477010 GLAST Thermo Fisher Scientific Cat #PA5-72895; RRID:AB_2718749 Goat anti-mouse (AlexaFluor 488) Invitrogen Cat #A-11029; RRID:AB_2534088 Goat anti-rabbit (AlexaFluor 594) Invitrogen Cat #A-11037; RRID:AB_2534095 Deposited data Mass Spectrometry data of hiPSCs-dervied brain organoids This paper Accession code: https://doi.org/10.5281/zenodo.17993221 Proteomic dataset of human fetal brain Melliou et al15 https://doi.org/10.1016/j.celrep.2022.110846 Mendeley Data, raw datas This paper Accession code: https://data.mendeley.com/datasets/9m8zzmdbb9/1 Experimental models: Cell lines BXS0115 American Type Culture Collection (ATCC) ACS-1029 lot number 0232 MAA3 Institute of Molecular Life Sciences at the HUN-REN Research Centre for Natural Sciences in Hungary, Agota Apati Sui001-A HYS0103 American Type Culture Collection (ATCC) ACS-1020 lot number 0176 Pk CEA Fontenay aux Roses/SupBiotech, France N/A SP CEA Fontenay aux Roses/SupBiotech, France N/A Software and algorithms BioRender BioRender https://www.biorender.com/ ImageJ ImageJ https://imagej.net/software/fiji/ GraphPad Prism v10 GraphPad Software https://www.graphpad.com/resources 526 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 527 Human iPSC generation and characterization 528 Reprogramming 529 Jo urn al Pr -pr oo f 19 All research involving human induced pluripotent stem cells (hiPSCs) was conducted in accordance 530 with the principles outlined in the ISSCR 2021 Guidelines. ..

    Software:

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxic in human brain organoids
    Article Snippet: 496 497 STAR METHODS 498 Detailed methods are provided in the online version of this paper and include the following: 499 • KEY RESOURCES TABLE 500 • RESOURCES AVAILABILITY 501 o Lead contact 502 o Materials availability 503 o Data and code availability 504 • EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 505 o Human iPSC generation and characterization – Reprogramming 506 o Human iPSC culturing 507 o Assessment of pluripotency 508 • METHOD DETAILS 509 o Cerebral organoids culture conditions 510 o Hypoxia modelling 511 o Immunofluorescence 512 o RNA extraction, RT-qPCR 513 o Shotgun proteomics 514 • QUANTIFICATION AND STATISTICAL ANALYSIS 515 o Proteomic Data Analysis 516 o Statistical analysis 517 518 519 Jo rna l P re- pro of 18 520 521 522 STAR METHODS 523 KEY RESOURCE TABLE 524 525 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SOX2 Abcam Cat #ab93689; RRID:AB_10562630. .. PAX6 Sigma-Aldrich Cat #AMAb91372; RRID:AB_2716656 NeuN Sigma-Aldrich Cat #MAB377; RRID:AB_2298772 MAP2 Sigma-Aldrich Cat #ZRB2290; RRID:AB_3677657 GFAP Sigma-Aldrich Cat #G3893; RRID:AB_477010 GLAST Thermo Fisher Scientific Cat #PA5-72895; RRID:AB_2718749 Goat anti-mouse (AlexaFluor 488) Invitrogen Cat #A-11029; RRID:AB_2534088 Goat anti-rabbit (AlexaFluor 594) Invitrogen Cat #A-11037; RRID:AB_2534095 Deposited data Mass Spectrometry data of hiPSCs-dervied brain organoids This paper Accession code: https://doi.org/10.5281/zenodo.17993221 Proteomic dataset of human fetal brain Melliou et al15 https://doi.org/10.1016/j.celrep.2022.110846 Mendeley Data, raw datas This paper Accession code: https://data.mendeley.com/datasets/9m8zzmdbb9/1 Experimental models: Cell lines BXS0115 American Type Culture Collection (ATCC) ACS-1029 lot number 0232 MAA3 Institute of Molecular Life Sciences at the HUN-REN Research Centre for Natural Sciences in Hungary, Agota Apati Sui001-A HYS0103 American Type Culture Collection (ATCC) ACS-1020 lot number 0176 Pk CEA Fontenay aux Roses/SupBiotech, France N/A SP CEA Fontenay aux Roses/SupBiotech, France N/A Software and algorithms BioRender BioRender https://www.biorender.com/ ImageJ ImageJ https://imagej.net/software/fiji/ GraphPad Prism v10 GraphPad Software https://www.graphpad.com/resources 526 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 527 Human iPSC generation and characterization 528 Reprogramming 529 Jo urn al Pr -pr oo f 19 All research involving human induced pluripotent stem cells (hiPSCs) was conducted in accordance 530 with the principles outlined in the ISSCR 2021 Guidelines. ..

    Stem Cell Culture:

    Article Title: Efficient and robust generation of functional hematopoietic cells from human pluripotent stem cells in albumin-free conditions.
    Article Snippet: .. Pluripotent stem cell culture All ESC and iPSC lines, WA09 (a property of WiCell), ACS-1020 (ATCC), ACS-1028 (ATCC), BC1,15 SCTi003-A (StemCell Technologies), HC4 and HC8,16 and PVB1.4,17 were maintained on six-well cell culture plates coated with Matrigel (Corning) in mTeSR Plus medium (StemCell Technologies) for routine maintenance. .. The cells were routinely passaged with 0.5 mM EDTA dissociation buffer when their confluency reached ∼70%-90%.18 Accutase (StemCell Technologies) was used for preparation for hematopoietic differentiation.

    Cell Culture:

    Article Title: Efficient and robust generation of functional hematopoietic cells from human pluripotent stem cells in albumin-free conditions.
    Article Snippet: .. Pluripotent stem cell culture All ESC and iPSC lines, WA09 (a property of WiCell), ACS-1020 (ATCC), ACS-1028 (ATCC), BC1,15 SCTi003-A (StemCell Technologies), HC4 and HC8,16 and PVB1.4,17 were maintained on six-well cell culture plates coated with Matrigel (Corning) in mTeSR Plus medium (StemCell Technologies) for routine maintenance. .. The cells were routinely passaged with 0.5 mM EDTA dissociation buffer when their confluency reached ∼70%-90%.18 Accutase (StemCell Technologies) was used for preparation for hematopoietic differentiation.



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